Our Mission:

Our Mission is to prepare students such that they function better and succeed in schools, colleges and universities. This is not happening now and we want to remedy this by preparing students via an integrative way of teaching that we developed. In this regard, consider us a special preparatory institution and a partner which prepares students so they succeed instead of failing while pursuing their education.

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What makes education difficult and what prevents learning and understanding?

There are two causes of this:
One is the subject based format of teaching which overly fragments knowledge which is further broken down into smaller pieces, often randomly, via the lecture format of delivery which happens more often when different professors are assigned to teach different parts of a given course, and

Two, the biggest obstacle to learning is a bit more serious and hidden than the one mentioned above; it is the knowledge left behind and not brought forward.

This omission produces gaps making it difficult for a student to understand what is being taught and why. A student thus opts for the memorization route, thereby compromising his or her potential settling for much less than he or she is capable of becoming.

We prevent this from happening by exposing students to an overview of what is known from our beginning to now and what is being done to expand it further. The groping and somewhat lost student thus finds his or her bearings and thereby becomes capable of growing with the growing and evolving society.

The unexpected part of this integrative way of teaching is that as we integrate knowledge, feeing it from much minutia and redundancies, it shrinks becoming less to teach with better understanding than the conventional way of prolonged teaching.

For instance, science, via this process of integration gets reduced to mere 150 concepts and skills which if learned first in a lab setting makes the rest of science, both basic and applied, easy to navigate through.

Same happens to the rest of our knowledge of art, humanities, psychology, sociology, anthropology and economics etc. They also shrinks and when their essence is merged with that of science, we end up with a holistic collage of practically the whole of our functional knowledge enabling us to become analytical, creative and versatile.

This form of integration also allows us to teach much by offering only six courses and a few workshops, and not the hundreds that the conventional schools or colleges teach. Much confusion that the students experience is thus eliminated.

This makes learning not only fast and easy but also comprehensible and less expensive. Students pursing higher education with this integrative background will have no difficulty completing their further education with flying colors and will stand much better hance for landing a job or entering the career they aspire for.

Our Catalogue

1. ORDERING INFORMATION

Items listed in this catalogue are provided for educational purposes and are not intended for use in clinical or other testing laboratories.

WE HELP YOU TEACH BETTER WITH LESS MONEY

We provide supplies only to bonafide institutes of learning.

All items in this catalogue are guaranteed classroom quality™. They are intended for teaching purposes only and are not to be used for clinical or other testing purposes.
How to place an order:
Using your official purchase order form or letterhead, please supply the following information about each item ordered:
  • Name of the product
  • Catalogue item number
  • Size
  • Quantity desired
  • Date delivery required
  • Price
Additionally, please provide your purchase order number, shipping and billing addresses and e-mail address. Mail completed orders to:

Educational Materials Division
Int. Inst. of Biochemical and Biomedical Technology
729 South Western Avenue
Chicago, Illinois, USA 60612-3532

OR

For expediency, fax orders via our 24 hours FAX line: (312) 243-2041 or e-mail to: info@iibbt.com

NOTE: We do not accept phone orders unless followed by an official faxed or mailed hard copy.

Credit:
We extend credit to all educational institutions that send their orders on their official purchase order form or letterhead and signed by an authorized individual from the institute. Such orders will be honored and shipped promptly. Payment for these orders is due when billed.
Prices and Invoicing: Prices are f.o.b. our address and are subject to change without notice. All billing is done at current prices plus shipping and handling charges. Handling charge is $10. Shipping charges will be as per prevailing UPS rates.

We accept payment by VISA and Master card also. Please include VISA or Master card number, expiration date, name and address of cardholder with zip code.

Guarantee:
Our products are Guaranteed Classroom Quality™ and they are unconditionally guaranteed. Items found unsatisfactory will be replaced. Please notify our Educational Materials Division before returning such items.

2. LAB SUPPLIES


Item # Item Name Quantity Price
MM-53 Acetone 1 gallon $51.00
MM-52 Alcohol reagent1 gallon $30.00
MM-39 Anaerobic incubation - gas pack for ea. $ 3.50
MM-40 Anaerobic incubation - anaerobic Indicator Strip ea. $ 1.00
MM-41 Anaerobic incubation – jar for ea. $250.00
MM-01 Applicator sticks plain 72 doz. box $12.00
MM-42 Autoclave Tape roll $ 5.50
MM-43 Bibulous Paper book of 50 $ 3.00
MM-44 CO2 incubation - Candle for ea. $ 1.00
MM-45 CO2 incubation – Jar for ea. $25.00
MM-02 Cotton – absorbent lb. $12.50
MM-03 Cotton – nonabsorbent lb. $22.50
MM-04 Cover slips 22x22 mm #1 oz. $4.50
MM-05 Detergents for glassware 5 lb $15.60
MM-06 Disinfectant 1 gallon $15.00
MM-07 Dropper bottles for staining - polyethylene 1 oz. ea. $1.75
MM-08 Filter paper 18.5 cm dia.pkg $25.00
MM-09 Funnel glass -versatile 100 mm takes 18.5 cm filter ea. $6.50
MM-10 Funnel plastic – 100mm takes 18.5 filter ea. $7.50
MM-11 Immersion Oil 4 oz. $6.00
MM-12 Inoculating handles for loops and needles ea. $18.00
MM-12 A Nichrome wire for above handles ea. $18.00
MM-12 B Needles, nichrome wire for above handles ea. $18.00
MM-12 C Loops, nichrome wire for above handles ea. $18.00
MM-13 Inoculating loops of nichrome wire with handles ea. $5.00
MM-14 Inoculating needles of nichrome wire with handles ea. $5.00
MM-15 Lens paper - book of 50 sheets ea. $1.00
MM-16 Lens cleaner ea. $5.50
MM-17 Membrane filters 25 mm dia. (0.22 micron), sterile disposable for filter sterilizing liquidsea. $2.00
MM-18 Membrane filter assembly, plastic of 47 mm dia Ea. $75.00
MM-20 Mounting fluid for permanent slides 4 oz. $7.00
MM-21 Petri dish disposable sterile (15 x 100 mm) 500 per box $90.00
MM-47 Petri dish disposable sterile (15 x 100 mm) 20 per sleeve(a)
25 per sleeve(b)
$6.00(a)
$7.00(b)
MM-48 Petri dish disposable sterile (15 x 150 mm) for Antibiotic Sensitivity Testing (The Kirby-Bauer Method) 10 per sleeve $ 20.00
MM-50 Pipettes sterile disposable (1.0 ml) ea. $ 0.40
MM-51 Pipettes sterile disposable (10 ml) ea. $ 0.65
MM-22 Plastic bags – autoclaveable for disposing infectious material 14" x 19" ea.
19" x 23" ea.
25" x 35" ea.
$0.35
$0.70
$1.10
MM-23 Slides, cavity 1 x 3" with single cavity ea. $10.00
MM-24 Slides, one end frosted 1/2 gross box $9.25
MM-25 Slides – plain 1/2 gross box $9.25
MM-26 Swabs sterile 100 ct. $10.00
MM-27 Teasing needles for teasing fungal specimen – straight ea. $2.00
MM-28 Teasing needles for teasing fungal specimen - angular point ea. $2.00
MM-29 Tongue depressor 500 per pkg $15.00
MM-30 Tooth pick pkg $2.50
MM-31 Vaseline 4 oz. $1.50
MM-32 Vaspar 4 oz. $10.00
MM-33 Wash bottles - 16 oz. Polyethylene ea. $4.00
MM-37 Wash bottles - 8 oz. Polyethylene ea. $4.00
MM-38 Wash bottles - 4 oz. Polyethylene ea. $4.00
MM-34 Wax marking pencil ea. $1.00
MM-35 Xylene 4 oz. $8.50
MM-36 Laboratory Manual ea. $30.00

5. MEDIA


MEDIA POWDER:
NOTE: To reduce waste we supply media in 50.0 grams quantities only. Media being hydroscopic tends to harden absorbing moisture from air. Keep lids tight all times.

MEDIA PREPARED:
While you can prepare your own media, a considerable amount of time and money can be saved by purchasing prepared media provided you limit the purchase of such media to what is actually needed. Even if you prepare your own media, do not prepare more than what is needed. Microbiological media, especially agar plates, have poor shelf life as they not only dry out but also tend to get contaminated with repeated handling.

For these reasons, this catalogue lists only those media which are essential for teaching a comprehensive course in microbiology. Prepared media are supplied, sterile, in tubes as slants, deeps, and broths and in standard size Petri plates as agar plates.

Certain media that are used as plates are also bulk packed in two size bottles. One size bottle has sufficient amount of the medium for preparing 15 plates. The other size has sufficient medium to prepare 5 plates. These bulk packs are especially useful for doing viable counts.

This is an economical way for preparing plates as the bulk packed medium can be melted and poured in plates as needed. If the entire contents of the bottle are not used, the leftover medium can still be saved and re-melted for preparing more plates. While such re-melted medium may not be suitable for performing clinical or other tests, it is perfectly acceptable for teaching purposes.

Occasionally also, when only a few plates are needed, agar medium supplied as deeps can be melted and poured into plates. For this reason each deep, as supplied, contains sufficient medium to pour one plate.

Directions for handling bulk packed medium or deeps for pouring plates:
All bulk packed medium is supplied in approximately 250 ml quantity in 500 ml screw capped bottles or in approximately 100 ml quantity in 250 ml screw capped bottles. Melt media contained in these bottles by immersing them, halfway, in a beaker of water and then bringing the water slowly to a boil. Keep boiling until the medium is fully melted. Cool medium to approximately 45 C and then pour plates.

Note: Besides being useful for pouring plates, this bulk packed solid medium is also useful for doing viable counts. For this purpose, medium packed in 100 ml quantities is preferred as this amount is sufficient to pour 5 plates.

NOTE: The Institute assumes no responsibility for breakage of bottles or tubes while melting agar media contained in them.

MICROBIOLOGICAL MEDIA – PRICES ARE AS SHOWN The following media are supplied sterile in screw cap tubes, bottles, or Petri plates. Minimum order is 10 tubes or plates. Order additional medium in increments of 10 also. No minimum order on bottled media.

Media marked with an * are special order media. These are prepared fresh upon receipt of order.
Item # Item Name Quantity Price
MP-01 Agar for solidifying media 50.0 g $20.00
MP-02 Blood agar base 50.0 g $15.00
MP-03 Brain Heart Infusion broth 50.0 g $20.00
MP-04 EMB Agar 50.0 g $22.00
MP-05 Heart Infusion broth 50.0 g $15.00
MP-06 Litmus milk 50.0 g $22.00
MP-07 MacConkey Agar 50.0 g $20.00
MP-08 Mannitol salt agar 50.0 g $18.00
MP-09 MRVP broth 50.0 g $20.00
MP-10 Mueller Hinton Agar 50.0 g $20.00
MP-11 Nitrate broth 50.0 g $10.00
MP-12 Nutrient agar 50.0 g $18.00
MP-13 Nutrient broth powder 50.0 g $18.00
MP-14 Phenol red broth for sugar fermentation tests 50.0 g $8.00
MP-15 Purple broth (brom cresol purple) 50.0 g $15.00
MP-16 Thioglycolate broth 50.0 g $18.00
MP-17 Trypticase soy broth 50.0 g $10.00
MP-18 Simmon’s Citrate agar 50.0 g $28.00
MP-19 Urea broth 50.0 g $18.00
MEDIA ADDITIVES:
MA-01 Gelatin 50.0 g $10.00
MA-02 Glucose (Dextrose) 50.0 g $10.00
MA-03 Lactose 50.0 g $10.00
MA-04 Lipase (fat reagent) 25.00 ml $10.00
MA-05 Starch 50.0 g $10.00
MA-06 Sucrose 50.0 g $10.00

5(a). BACTERIOLOGICAL MEDIA


Item # Item Name Quantity Price
PM-62 Acetamide agar for acetamide hydrolysis * ea. $1.50
PM-77 Azide dextrose broth ea. $0.85
PM-63 Bile esculin agar slants ea. $0.75
PM-01 Blood agar slant ea. $1.50
PM-02 Blood agar plate ea. $1.25
PM-03 BHI (Brain Heart Infusion) broth ea. $0.80
PM-04 BHI agar slant ea. $0.80
PM-05 BHI agar plate ea. $0.90
PM-06 BHI agar deep ea. $0.85
PM-07 BHI agar bottle 250 ml quantity ea. $20.00
PM-08 BHI agar bottle 100 ml quantity ea. $15.00
PM-09 BHI broth with 6.5% sodium chloride ea. $0.75
PM-10 BCP glucose (Brom Cresol Purple) broth with gas vial ea. $1.25
PM-11 BCP lactose broth with gas vial ea. $1.25
PM-64 BCP Maltose broth with vial ea. $1.25
PM-12 BCP sucrose broth with gas vial ea. $1.25
PM-13 Brilliant green lactose bile broth with gas vial ea. $1.25
PM-14 Chocolate agar plate ea. $2.50
PM-15 Chocolate agar slant - NO LONGER AVAILABLE ea. $1.25
PM-16 CTA glucose (Cystine Trypticase Agar) semisolid stab ea. $0.75
PM-17 CTA lactose semisolid stab ea. $0.75
PM-18 CTA sucrose semisolid stab ea. $0.75
PM-19 CTA maltose semisolid stab ea. $0.75
PM-65 Decarboxylase broth with arginine ea. $0.80
PM-78 Decarboxylase broth with lysine ea. $0.80
PM-79 Decarboxylase broth with ornithine ea. $0.80
PM-20 Dextrose-starch agar plate ea. $0.85
PM-21 DNAse agar plate ea. $0.85
PM-22 Egg yolk agar for lecithinase activity ea. $1.00
PM-23 Eugone Agar plate* ea. $1.50
PM-24 EMB (Eosin Methylene Blue) agar plate ea. $0.85
PM-25 Endo broth ea. $1.00
PM-80 F M agar for fusobacterium* ea. $1.50
PM-26 Fluorescein agar slant ea. $0.75
PM-66 Galatin agar plate for gelatine hydrolysis ea. $0.85
PM-67 Gluconate peptone broth ea. $0.75
PM-95 Hippurate broth ea. $0.75
PM-96 KF broth detects group D strep from non D.* Also see SF broth ea. $1.50
PM-27 Kligler's iron agar slant ea. $0.80
PM-28 Lactose broth single strength with gas vial ea. $1.25
PM-29 Lactose broth double strength with gas vial ea. $1.25
PM-81 Letheen agar for environmental testing * ea. $1.50
PM-107 Letheen broth ea. $1.00
PM-30 Litmus milk broth tube ea. $0.85
PM-68 Lysine iron agar slants ea. $0.85
PM-69 Malonate broth ea. $0.75
PM-31 Mannitol salt agar plate ea. $0.85
PM-32 Milk agar slant ea. $0.75
PM-33 Motility test medium ea. $0.75
PM-34 MRVP (Methyl red-Voges-Proskauer) broth ea. $0.75
PM-35 MacConkey agar plate ea. $0.85
PM-36 Mueller Hinton agar plate ea. $0.85
PM-37 Nitrate broth ea. $0.75
PM-38 Nutrient broth ea. $0.75
PM-39 Nutrient agar slant ea. $0.75
PM-40 Nutrient Agar deep ea. $0.85
PM-41 Nutrient agar plate ea. $0.85
PM-42 Nutrient agar bottle-250 ml quantity ea. $25.00
PM-43 Nutrient agar bottle-100 ml quantity ea. $20.00
-
O-F (Oxidation-Fermentation medium with brom thymol blue (BTB) indicator for enterics*
- -
PM-70 O-F Glucose for enterics * ea. $1.50
PM-71 O-F Sucrose for enterics * ea. $1.50
PM-72 O-F Lactose for enterics * ea. $1.50
PM-97 O-F Maltose for enterics * ea. $1.50
PM-98 O-F Mannitol for enterics * ea. $1.50
PM-99 O-F Xylose for enterics * ea. $1.50
-
O-F medium with brom cresol purple (BCP) Indicator for Staphylococci*
- -
PM-100 O-F Glucose for Staph * ea. $1.50
PM-101 O-F Mannitol for Staph * ea. $1.50
PM-102 ONPG broth for beta galactosidase detection * ea. $1.50
PM-82 Orange serum agar for lactobacilli and fungi * ea. $1.50
PM-73 Phenylalanine agar slant ea. $0.60
PM-44 Pyocyanin agar slant ea. $0.60
PM-83 Photobacterium agar slant ea. $1.50
PM-84 Selenite cystine enrichment broth ea. $0.60
PM-103 SF broth detects group D enterococci from nonenterococci group D * ea. $1.50
PM-45 Simmons citrate agar slant ea. $0.75
PM-85 Snyder agar for oral lactobacilli * ea. $1.50
PM-74 Sodium hippurate broth ea. $0.75
PM-46 Spirit Blue agar plate (for detecting lipolysis - fat hydrolysis) ea. $1.20
PM-47 SS (Salmonella-Shigella) agar plate ea. $0.85
PM-75 Starch agar plates for starch hydrolysis test ea. $0.85
PM-86 Tetrathionate enrichment broth ea. $0.90
PM-48 Thioglycolate broth ea. $0.90
PM-76 Triple sugar iron agar slant ea. $1.00
PM-49 TS (Trypticase soy) broth ea. $0.80
PM-50 TS agar slant ea. $0.80
PM-51 TS agar deep ea. $0.80
PM-52 TS agar plate ea. $0.90
PM-53 TS agar bottle 250 ml quantity ea. $25.00
PM-54 TS agar bottle 100 ml quantity ea. $20.00
PM-55 Tryptone broth for indole test ea. $0.75
PM-56 Urea broth ea. $1.00
PM-87 Veillonella agar* ea. $1.50
PM-88 XLD agar plate ea. $1.00
-
MEDIA FOR GROWING FUNGI (MOLDS AND YEASTS) AND ACTINOMYCETES
- -
PM-89 Actinomycete isolation agar plate ea. $1.00
PM-106 Corn meal agar slant ea. $1.00
PM-90 Cornmeal agar plate ea. $1.25
PM-104 Corn meal agar plates with Tween 80* ea. $1.50
PM-57 Malt extract agar slant ea. $0.75
PM-58 Potato dextrose agar slant ea. $0.75
PM-59 Potato dextrose agar plate ea. $0.85
PM-60 Sabouraud dextrose agar slant ea. $0.75
PM-61 Sabouraud dextrose agar plate ea. $0.85
PM-91 Trichophyton agar #4 plate * ea. $1.50
PM-92 Trichophyton agar slant * ea. $1.50
PM-93 Wolin Bevis agar for Candida chlamydospore production * ea. $1.50
-
SPECIAL REAGENTS OCCASSIONALLY NEEDED TO BE USED WITH MEDIA:
- -
PM-94 Mineral oil – sterile for overlay (10.0 ml) ea. $2.00
PM-105 Vaspar - sterile for overlay (4 oz) ea. $10.00

6. CULTURES


While there are an overwhelming number of cultures, one needs only a few, in most cases nonpathogenic ones, to teach the essential principles of microbiology. Nonetheless, the list on the following pages is rather extensive and is provided so that different instructors can select cultures of their choice.

We would, however, like to emphasize that keeping too many unnecessary cultures in a teaching laboratory is time consuming, as they need to be transferred frequently and also their purity has to be tested at frequent intervals. This time can be saved by keeping only the essential cultures and limiting one's stock of cultures to the hardier ones rather than those that die rapidly when maintained in the laboratory.

It should also be noted that live cultures are not always necessary for teaching principles of microbiology. Procedures designed to demonstrate morphology and staining reactions such as the Gram stain and cytological stains can be performed on killed cultures. Live cultures are essential only for biochemical reactions and for growth experiments. Immunological and serological reactions also do not

require live cultures. Killed cultures are the cultures of choice here, and this catalogue provides such cultures. These are easy to keep and since they have a longer shelf life, they are economical to use. Using the killed cultures as opposed to live cultures can thus save a considerable amount of cost.

The use of the killed cultures also enables the students and staff to be less apprehensive about working with microbes, especially since the connotation "germs" is associated with disease production. Such cultures would, therefore, enable you to add lab based microbiology courses at introductory college or even at high school level. NOTE:
We regard most living cultures as potentially pathogenic and ship these only to colleges and universities. Cultures that we ship to high schools are marked with an asterisk. We do not ship cultures to individuals. All bacterial and yeast cultures are available as live cultures on slants and also as killed suspensions. All fungal and actinomycetes cultures are available as live cultures on slants and also as killed mycelial mass. The latter is pre-stained, ready to tease, mount, and observe.
Live Item # Killed Item # Item Quantity Live(slant) price Live(plate) price Killed price
LB-01 KB-01 Aeromonas hydrophilia One $5.00 $10.00 $10.00
LB-02 KB-02 Alcaligenes faecalis One $5.00 $10.00 $10.00
LB-03 KB-03 Alcaligenes viscolactis One $5.00 $10.00 $10.00
LB-04 KB-04 Arizona hinshawii One $5.00 $10.00 $10.00
LB-05 KB-05 Bacillus alvei One $5.00 $10.00 $10.00
LB-06 KB-06 Bacillus cereus* One $5.00 $10.00 $10.00
LB-07 KB-07 Bacillus cereus var. mycoides One $5.00 $10.00 $10.00
LB-08 KB-08 Bacillus coagulans One $5.00 $10.00 $10.00
LB-09 KB-09 Bacillus megaterium* One $5.00 $10.00 $10.00
LB-10 KB-10 Bacillus polymyxa One $5.00 $10.00 $10.00
LB-11 KB-11 Bacillus stearothermophilus One $5.00 $10.00 $10.00
LB-12 KB-12 Bacillus subtilis* One $5.00 $10.00 $10.00
LB-13 KB-13 Bacillus subtilis var. globigii One $5.00 $10.00 $10.00
LB-14 KB-14 Bacteroides fragilis One $5.00 $10.00 $10.00
LB-15 KB-15 Brenhamella catarrhalis (previously Neisseria catarrhalis) One $5.00 $10.00 $10.00
LB-16 KB-16 Chromobacterium violaceum One $5.00 $10.00 $10.00
LB-17 KB-17 Citrobacter freundii (previously Escherichia freundii) One $5.00 $10.00 $10.00
LB-18 KB-18 Citrobacter intermedius One $5.00 $10.00 $10.00
LB-19 KB-19 Clostridium botulinum One $5.00 $10.00 $10.00
LB-20 KB-20 Clostridium butyricum One $5.00 $10.00 $10.00
LB-21 KB-21 Clostridium difficile One $5.00 $10.00 $10.00
LB-22 KB-22 Clostridium histolyticum One $5.00 $10.00 $10.00
LB-23 KB-23 Clostridium perfringens One $5.00 $10.00 $10.00
LB-24 KB-24 Clostridium sporogenes One $5.00 $10.00 $10.00
LB-25 KB-25 Clostridium tetani One $5.00 $10.00 $10.00
LB-26 KB-26 Corynebacterium diphtheriae One $5.00 $10.00 $10.00
LB-27 KB-27 Corynebacterium pseudodiphtheriticum* One $5.00 $10.00 $10.00
LB-28 KB-28 Corynebacterium xerosis One $5.00 $10.00 $10.00
LB-29 KB-29 Edwardsiella tarda One $5.00 $10.00 $10.00
LB-30 KB-30 Enterobacter aerogenes (previously Aerobacter aerogenes) One $5.00 $10.00 $10.00
LB-31 KB-31 Enterobacter cloacae One $5.00 $10.00 $10.00
LB-32 KB-32 Escherichia coli* One $5.00 $10.00 $10.00
LB-33 KB-33 Escherichia coli Strain B* One $5.00 $10.00 $10.00
LB-34 KB-34 Escherichia coli (gene transfer donor) One $5.00 $10.00 $10.00
LB-35 KB-35 Escherichia coli (gene transfer recipient) One $5.00 $10.00 $10.00
LB-36 KB-36 Flavobacterium capsulatum* One $5.00 $10.00 $10.00
LB-37 KB-37 Gaffkya tetragena One $5.00 $10.00 $10.00
LB-38 KB-38 Hafnia alvei One $5.00 $10.00 $10.00
LB-39 KB-39 Halobacterium salinarium One $5.00 $10.00 $10.00
LB-40 KB-40 Klebsiella pneumoniae One $5.00 $10.00 $10.00
LB-41 KB-41 Lactobacillus acidophilus One $5.00 $10.00 $10.00
LB-42 KB-42 Lactobacillus casei One $5.00 $10.00 $10.00
LB-43 KB-43 Leuconostoc mesenteroides One $5.00 $10.00 $10.00
LB-44 KB-44 Listeria monocytogenes One $5.00 $10.00 $10.00
LB-45 KB-45 Micrococcus luteus* (previously Sarcina lutea) One $5.00 $10.00 $10.00
LB-46 KB-46 Morganella morganii (previously Proteus Morganii) One $5.00 $10.00 $10.00
LB-47 KB-47 Mycobacterium smegmatis One $5.00 $10.00 $10.00
LB-48 KB-48 Mycobacterium phlei* One $5.00 $10.00 $10.00
LB-49 KB-49 Mycobacterium tuberculosis One $5.00 $10.00 $10.00
LB-50 KB-50 Neisseria subflava One $5.00 $10.00 $10.00
LB-51 KB-51 Neisseria sicca One $5.00 $10.00 $10.00
LB-52 KB-52 Photobacterium fischeri One $5.00 $10.00 $10.00
LB-53 KB-53 Proteus mirabilis One $5.00 $10.00 $10.00
LB-54 KB-54 Proteus vulgaris One $5.00 $10.00 $10.00
LB-55 KB-55 Pseudomonas aeruginosa One $5.00 $10.00 $10.00
LB-56 KB-56 Pseudomonas cepacia One $5.00 $10.00 $10.00
LB-57 KB-57 Pseudomonas fluorescens One $5.00 $10.00 $10.00
LB-58 KB-58 Pseudomonas putida One $5.00 $10.00 $10.00
LB-59 KB-59 Providencia rettgeri One $5.00 $10.00 $10.00
LB-60 KB-60 Providencia stuartii One $5.00 $10.00 $10.00
LB-61 KB-61 Rhodococcus rhodochrous One $5.00 $10.00 $10.00
LB-62 KB-62 Rhodospirillum rubrum* One $5.00 $10.00 $10.00
LB-63 KB-63 Salmonella cholerae-suis One $5.00 $10.00 $10.00
LB-64 KB-64 Salmonella enteritidis One $5.00 $10.00 $10.00
LB-65 KB-65 Salmonella montevideo One $5.00 $10.00 $10.00
LB-66 KB-66 Salmonella paratyphi A One $5.00 $10.00 $10.00
LB-67 KB-67 Salmonella gallinarum One $5.00 $10.00 $10.00
LB-68 KB-68 Salmonella schottmuelleri (Salmonella paratyphi B) One $5.00 $10.00 $10.00
LB-69 KB-69 Salmonella typhimurium One $5.00 $10.00 $10.00
LB-70 KB-70 Salmonella typhosa One $5.00 $10.00 $10.00
LB-71 KB-71 Sarcina lutea (see Micrococcus luteus) One $5.00 $10.00 $10.00
LB-72 KB-72 Serratia marcescens* One $5.00 $10.00 $10.00
LB-73 KB-73 Shigella boydii One $5.00 $10.00 $10.00
LB-74 KB-74 Shigella dysenteriae One $5.00 $10.00 $10.00
LB-75 KB-75 Shigella flexneri One $5.00 $10.00 $10.00
LB-76 KB-76 Shigella sonnei One $5.00 $10.00 $10.00
LB-77 KB-77 Spirillum itersonii One $5.00 $10.00 $10.00
LB-78 KB-78 Spirillum serpens* One $5.00 $10.00 $10.00
LB-79 KB-79 Staphylococcus epidermidis* One $5.00 $10.00 $10.00
LB-80 KB-80 Staphylococcus aureus One $5.00 $10.00 $10.00
LB-81 KB-81 Staphylococcus aureus (for CAMP test) One $5.00 $10.00 $10.00
LB-82 KB-82 Staphylococcus saprophiticus* One $5.00 $10.00 $10.00
LB-83 KB-83 Streptococcus pyogenes, beta hemolytic - group A One $5.00 $10.00 $10.00
LB-84 KB-84 Streptococcus agalactiae, beta hemolytic - group B One $5.00 $10.00 $10.00
LB-85 KB-85 Streptococcus equi, beta hemolytic - group C One $5.00 $10.00 $10.00
LB-86 KB-86 Streptococcus faecalis, gamma hemolytic - group D One $5.00 $10.00 $10.00
LB-87 KB-87 Streptococcus faecalis, var liquifaciens - gamma hemolytic - group D One $5.00 $10.00 $10.00
LB-88 KB-88 Streptococcus bovis, alpha to gamma occasionally beta hemolytic - group D One $5.00 Live Killed
LB-89 KB-89 Streptococcus equinus, alpha hemolytic - group D One $5.00 $10.00 $10.00
LB-90 KB-90 Streptococcus sanguis, alpha hemolytic - group H One $5.00 $10.00 $10.00
LB-91 KB-91 Streptococcus salivarius, gamma hemolytic - group K One $5.00 $10.00 $10.00
LB-92 KB-92 Streptococcus lactis, alpha hemolytic - group N One $5.00 $10.00 $10.00
LB-93 KB-93 Streptococcus mutans, alpha to gamma hemolytic - cariogenic One $5.00 $10.00 $10.00
LB-94 KB-94 Streptococcus pneumoniae (Previously Pneumococcus pneumonia)alpha hemolytic - group None One $5.00 $10.00 $10.00
LB-95 KB-95 Streptococcus mitis, alpha hemolytic - group None One $5.00 $10.00 $10.00
PS-38 - TB positive sputum smears. These are supplied unstained and are prepared from sterilized known positive TB sputum One $1.50 $10.00 $10.00
KB-96 - Vibrio cholera - available only as a killed suspension One - $10.00 $10.00
LB-97 KB-97 Vibrio fischeri One $5.00 $10.00 $10.00
- -
CULTURES OF ACTINOMYCETES - LIVE OR KILLED
- - - -
LA-98 KA-98 Streptomyces griseus One - $7.50 $20.00
- -
CULTURES OF FUNGI (MOLDS AND YEAST) - LIVE OR KILLED
- - - -
LF-99 KF-99 Alternaria sp One - $7.50 $20.00
LF-100 KF-100 Aspergillus niger* One - $7.50 $20.00
LF-101 KF-101 Candida albicans One - $7.50 $20.00
LF-102 KF-102 Candida parapsilosis One - $7.50 $20.00
LF-103 KF-103 Candida pseudotropicalis One - $7.50 $20.00
LF-104 KF-104 Candida tropicalis One - $7.50 $20.00
LF-105 KF-10 Epidermophyton floccosum One - $7.50 $10.00
LF-106 KF-106 Geotrichum candidum One - $7.50 $10.00
LF-107 KF-107 Microsporum canis One - $7.50 $10.00
LF-108 KF-108 Microsporum gypseum One - $7.50 $10.00
LF-109 KF-109 Mucor genevensis One - $7.50 $20.00
LF-110 KF-110 Mucor mucedo One - $7.50 $20.00
LF-111 KF-11 Penicillium camemberti One - $7.50 $20.00
LF-112 KF-112 Penicillium chrysogenum One - $7.50 $20.00
LF-113 KF-113 Penicillium notatum* One - $7.50 $20.00
LF-114 KF-114 Penicillium roqueforti One - $7.50 $20.00
LF-115 KF-115 Rhizopus nigricans plus strain* One - $7.50 $10.00
LF-116 KF-116 Rhizopus nigricans minus strain* One - $7.50 $10.00
LF-117 KF-117 Saccharomyces cerevisiae* One - $7.50 $20.00
LF-118 KF-118 Saccharomyces ellipsoideus One - $7.50 $20.00
LF-119 KF-119 Trichophyton rubrum One - $7.50 $10.00
LF-120 KF-120 Trichophyton mentagrophytes One - $7.50 $10.00
LF-121 KF-121 Trichophyton tonsurans One - $7.50 $10.00
LF-122 KF-122 Torula lactosa One - $7.50 $20.00
LF-123 KF-123 Torula rosea One - $7.50 $20.00
LF-124 KF-124 Rhizopus stolonifer plus strain* One - $7.50 $10.00
LF-125 KF-125 Rhizopus stolonifer minus strain* One - $7.50 $10.00

7. PREPARED SLIDES


Prepared slides have a special place in teaching microbiology for they enable a student to acquire an appreciation of cellular structure. Cytological studies of this nature also enable a student to develop an ability to see detail, to identify special structures and to relate structure with function. This form of exercise thus enhances comprehension and prepares one for further scientific pursuits. Furthermore, when a student eventually prepares his or her own slides, the experience gained with prepared slides enables the student to judge and improve his or her technique. Manual dexterity and neatness gained through such exercises is extremely vital for becoming a good scientist.
Item # Item Name Quantity Price
-
BACTERIAL SLIDES
--
-
Bacterial smears showing typical shape and arrangement
- -
PS-01 Bacillus cereus ea. $2.50
PS-02 Bordetella pertussis ea. $2.50
PS-03 Branhamella catarrhalis (previously Neisseria catarrhalis) ea. $2.50
PS-04 Brucella abortus ea. $2.50
PS-05 Clostridium tetani ea. $2.50
PS-06 Clostridium perfringens ea. $2.50
PS-07 Corynebacterium diphtheriae ea. $2.50
PS-08 Escherichia coli ea. $2.50
PS-09 Haemophilus influenza ea. $2.50
PS-10 Lactobacillus casei ea. $2.50
PS-11 Legionella pneumophilia ea. $5.50
PS-12 Listeria monocytogenes ea. $2.50
PS-13 Micrococcus luteus (previously Sarcina lutea) ea. $2.50
PS-14 Mycobacterium tuberculosis ea. $2.50
PS-15 Mixed bacterial smear showing coccus, bacillus and spiral shapes ea. $2.50
PS-16 Neisseria gonorrhoeae ea. $2.50
PS-17 Pasteurella species ea. $2.50
PS-18 Pseudomonas aeruginosa ea. $2.50
PS-19 Proteus vulgaris ea. $2.50
PS-20 Salmonella typhosa ea. $2.50
PS-21 Shigella dysenteriae ea. $2.50
PS-22 Spirillum serpens ea. $2.50
PS-23 Staphylococcus aureus ea. $2.50
PS-24 Staphylococcus epidermidis ea. $2.50
PS-25 Streptococcus pyogenes ea. $2.50
PS-26 Streptococcus pneumoniae (previously Diplococcus pneumoniae) ea. $2.50
PS-27 Streptococcus lactis ea. $2.50
PS-28 Treponema pallidum ea. $10.50
PS-29 Vibrio cholera ea. $5.50
-
Bacterial smears showing selected cytology
- -
-
Spore stain
- -
PS-30 Bacillus subtilis stained with malachite green to show spores ea. $3.50
PS-31 Bacillus subtilis stained with methylene blue to show unstained spores ea. $3.50
-
Capsule stain
- -
PS-32 Klebsiella pneumoniae showing capsule stained with Maneval's solution ea. $3.50
PS-33 Streptococcus pneumoniae showing capsules stained with Maneval's solution ea. $4.50
- Flagella Stain - -
PS-34
Bacterial cells showing flagella
ea. $10.50
-
Metachromatic granules
- -
PS-35 Corynebacterium diphtheria stained with Albert's stain ea. $5.50
-
Acid-fast stain
- -
PS-36 Acid-fast stain showing Mycobacterium tuberculosis mixed with non acid-fast bacteria ea. $5.50
PS-37 TB Positive sputum smear stained with acid-fast stain ea. $5.50
PS-38 TB Positive sputum smear unstained ea $1.75
-
Cell wall stain
- -
PS-39 Bacillus subtilis stained to show cell wall ea. $8.50
PS-40 Escherichia coli stained to show cell wall ea. $8.50
-
Fat granules
- -
PS-41 Bacillus subtilis stained to show fat granules ea $6.50
-
FUNGAL SLIDES (MOLDS ANDE YEASTS)
- -
PS-42 Aspergillus niger ea. $5.50
PS-43 Penicillium notatum ea. $5.50
PS-44 Rhizopus nigricans ea. $5.50
PS-45 Saccharomyces cereviseae ea. $5.50
PS-46 Trichophyton mentagrophytes ea $10.50
PS-47 Trichophyton rubrum ea $10.50
PS-48 Microsporum canis ea $10.50
PS-49 Microsporum gypseum ea $10.50
PS-50 Epidermophyton fluccosum ea $10.50
PS-51 Sporothrix schenckii ea $12.50
PS-52 Coccidioides immitis ea $12.50
PS-53 Histoplasma capsulatum ea $12.50
PS-54 Blastomyces dermatitidis ea $12.50
PS-55 Candida albicans - yeast phase ea $4.00
PS-56 Candida albicans - chlamydospores ea $12.50
-
ACTINOMYCETES SLIDES
- -
PS-57 Actinomyces bovis ea $10.50
PS-58 Actinomyces israelii ea $10.50
PS-59 Actinomyces viscosus ea $10.50
PS-60 Nocardia asteroides ea $10.50
PS-61 Streptomyces griseus ea $10.50

8. PREPARED STAIN REAGENTS


The following stains and staining reagents are supplied prepared, ready to use in screw cap bottles. Prices and quantities are as shown. A booklet describing selected staining procedures is also available at $5.00 each.
Item # Item Name Quantity Price
-
ACID FAST STAINING
- -
-
Ziehl-Neelsen's method
- -
SR-01 Ziehl-Neelsen carbol fuchsin 4 oz. $8.00
SR-02 Acid alcohol 8 oz. $10.00
SR-03 Loeffler's mehylene blue 4 oz. $6.00
-
Kinyoun's method
- -
SR-04 Kinyoun's carbol fuchsin 4 oz. $10.00
SR-02 Acid alcohol for decolorizing 8 oz. $10.00
SR-03 Loeffler's methylene blue 4 oz. $6.00
-
CAPSULE STAINING
- -
-
Maneval's method
- -
SR-05 Congo red solution 2 oz. $8.00
SR-06 Maneval's solution 4 oz. $15.00
-
Novelli's method
- -
SR-07 Alcian blue 4 oz. $10.00
SR-01 Ziehl-Neelsen's carbol fuchsin (same as for acid fast stain 4 oz. $8.00
-
Tyler’s modification of Anthony's method
- -
SR-08 Acetic acid-crystal violet 4 oz $8.00
SR-09 Copper sulfate solution 4 oz $8.00
-
Muir's method
- -
SR-01 Ziehl-Neelsen's carbol fuchsin 4 oz $8.00
SR-10 Muir's mordant 4 oz $15.00
SR-03 Loeffler's methylene blue 4 oz $6.00
-
CELL WALL STAINING
- -
-
Dyar's method
- -
SR-11 Cetyl pyridinium chloride 4 oz $20.00
SR-05 Congo red (same as for capsule stain by the Maneval's method) 2 oz. $8.00
-
Robinow's method
- -
SR-12 Tannic acid 4 oz $8.00
SR-13 Crystal violet solution (0.01%, aqueous) 4 oz $6.00
-
Hale's method
- -
SR-14 Phosphomolybdic acid 4 oz $20.00
SR-15 Methyl green solution 4 oz $8.00
-
Tomcsik and Grace's method
- -
SR-16 Alcian blue ethanolic solution 4 oz $10.00
SR-01 Ziehl-Neelsen's carbol fuchsin (same as for acid fast stain) 4 oz. $8.00
-
CYTOPLASMIC MEMBRANE STAINING
- -
-
Knaysi's method
- -
SR-17 Knaysi's mordant 4 oz $20.00
SR-01 Ziehl-Neelsen's carbol fuchsin (same as for acid fast stain) 4 oz. $8.00
-
GRAM STAINING
- -
SR-18 Gram’s Crystal violet solution 8 oz. $6.00
SR-19 Gram's iodine solution 8 oz. $6.00
SR-20 Acetone alcohol 8 oz. $6.00
SR-21 Safranin solution 8 oz. $6.00
-
FAT DROPLET STAINING
- -
-
Burdon's method
- -
SR-22 Sudan black B solution 2 oz. $15.00
SR-23 Safranin solution, 0.25% aqueous 4 oz. $8.00
-
FLAGELLA STAINING
- -
-
Leifson's method
- -
SR-24 Leifson's flagella stain (supplied as dry powder - prepare fresh just before use) 2 gm $15.00
SR-03 Loeffler's methylene blue 4 oz. $6.00
-
METACHROMATIC GRANULES STAINING
- -
-
Albert's method
- -
SR-25 Albert's staining solution 4 oz. $10.00
SR-26 Lugol's iodine - modified 4 oz. $8.00
-
Methylene blue method
- -
SR-27 Dilute alcoholic solution of methylene blue 4 oz. $10.00
SR-28 Sulfuric acid solution 1% 4 oz. $7.00
-
NUCLEAR MATERIAL STAINING
- -
-
Robinow's method
- -
SR-29 Hydrochloric acid IN 4 oz. $7.00
SR-30 Giemsa stain 4 oz. $20.00
SR-31 Basic fuchsin (aqueous) 0.3% 4 oz. $7.00
SR-32 Osmium tetroxide (supplied as dry crystals - prepare fresh before use) – work under hood, protect eyes from vapors 1/2 gm. $50.00
SR-33 Schaudinn's fixative 4 oz. $15.00
SR-34 Bouin's fixative 4 oz. $15.00
-
SPORE STAINING
- -
-
Negative staining method
- -
SR-03 Loefflers Methylene blue solution 4 oz. $6.00
-
Kinton's staining method
- -
SR-04 Kinyoun's carbol fuchsin (same as for acid fast stain) 4 oz. $10.00
SR-35 Alcohol 4 oz. $6.00
SR-03 Loeffler's Methylene blue solution - 4 oz. $6.00
-
Wirtz method
- -
SR-36 Malachite green solution 4 oz. $8.00
SR-21 Safranin (same as for Gram stain) 4 oz. $6.00
-
SIMPLE AND NEGATIVE STAINING OF BACTERIA
- -
-
Simple staining method
- -
SR-18 Crystal violet solution 8 oz. $8.00
-
Negative, relief of indirect staining method
- -
SR-37 Nigrosine solution (bacteriological grade) 4oz. $20.00
-
FUNGAL STAINS
- -
-
Lactophenol cottton blue method
- -
SR-38 Lactophenol cotton blue stain 2 oz. $15.00
SR-39 Clear mounting fluid 2 oz. $15.00
SR-40 One-step staining and mounting fluid for permanent mounts - contains mounting fluid and lactophenol cotton blue) 2 oz. $20.00
SR-41 BOOKLET DESCRIBING STAINING PROCEDURES ea. $5.00
SR-42 SELECTED STAINING PROCEDURES ea. $15.00

9. BIOCHEMICAL TESTS REAGENTS


Biochemical tests are the heart of microbiological laboratory procedures. These teach not only the basis of microbial identification but also Chemistry and Biochemistry. From the philosophical standpoint, these procedures reveal that life is possible with varying degrees of biochemical and biological diversity. Appreciating these parameters of life thus enhances a student's understanding of life. Additionally, these procedures help students understand their own nutrition and thus aid in healthful living.

These procedures are also important for understanding genetic engineering for it is these procedures which help select strains with biochemical traits of special interest. Markers which may aid pathogenesis are also studied via similar procedures.

BIOCHEMICAL TEST REAGENTS – PREPARED READY TO USE
The following reagents for biochemical tests are supplied prepared, ready to use in screw cap bottles. Prices and quantities are as shown. A booklet describing selected biochemical tests is also available at $ 5.00 each. See below.
Item # Item Name Quantity Price
-
Bacitracin sensitivity test (for identifying group A beta hemolytic streptococci)
--
BR-01 Bacitracin disks - vial of 50 discs ea. $20.00
-
Bile solubility test reagent
--
BR-02 Sodium taurocholate or bile solution 4 oz. $8.00
-
Catalase test reagent
--
BR-03 Hydrogen peroxide solution 3% 4 oz. $8.00
-
Coagulase test reagent
--
BR-04 Sterile plasma - reconstitute per bottle directions ea. $20.00
-
DNAse test reagent
--
BR-05 Hydrochloric acid solution 4 oz. $8.00
-
Gelatin hydrolysis test reagent
--
BR-06 Acidified mercuric chloride 4 oz. $20.00
-
Gluconate oxidation reagent
--
BR-21 Benedict’s solution 2 oz. $15.00
-
Hippurate hydrolysis reagent
--
BR-22 12% acidified ferric chloride 4 oz. $10.00
-
Indole test reagent
--
BR-07 Kovac's reagent 4 oz. $15.00
-
Mehyl red test reagent
--
BR-08 Methyl red solution 4 oz. $10.00
-
Nitrate reduction test reagents
--
BR-09 Sulfanilic acid 4 oz. $10.00
BR-10 N,N-dimethy1-a-naphthylamine(replaces a-naphthylamine which has been shown to be carcinogenic) 4 oz. $20.00
BR-11 Zinc dust suspension 1 oz. $5.00
-
Optochin test reagent
--
BR-12 Optochin disks - vial of 50 disks ea. $20.00
-
Oxidase test reagent
--
BR-13 Para-amino-dimethyl-aniline oxalate 2 oz. $15.00
-
Phenylalanine deaminase test reagent
--
BR-14 Acidic ferric chloride solution 4 oz. $10.00
-
Phosphatase test reagent
--
BR-15 Ammonium hydroxide 2oz. $8.00
-
Starch hydrolysis test reagent
--
BR-16 Modified Lugol's iodine solution 4 oz. $10.00
-
Voges-Proskauer test (VP test) reagent
--
BR-17 Potassium hydroxide solution 40% 4 oz. $8.00
BR-18 Alpha-naphthol 4 oz. $12.00
BR-19 BOOKLET DESCRIBING SELECTED BIOCHEMICAL TEST PROCEDURES ea. $5.50

10. RAPID AND MULTITEST ID SYSTEMS


MATERIALS FOR RAPID BIOCHEMICAL IDENTIFICATION OF MICROORGANISMS
Since 1960 emphasis has been shifting to rapid identification of micro-organisms using micro techniques and multiple test procedures.

Conventional procedures using media contained in test tubes and Petri plates were found to be too cumbersome, time consuming and uneconomical. Consequently, mini methods using just one isolated colony as the inoculum were developed. These methods speed up the identification procedures and provide diagnostic information in less time than the conventional methods. They also use less space and are standardized so that persons with a limited training can perform these procedures on a routine basis.

The first such method which became popular was the Enterotube method. Subsequently, other methods such as the API followed.

Concurrently, methods using multiple inoculating devices were also introduced. These methods have the advantage in that they use conventional procedures and, therefore, do not require retraining of personnel.

Since rapid test procedures are becoming more popular and are likely to be the methods of the future, it is suggested that a good microbiology course include, at least as demonstrations, some of these rapid and multitest procedures.
Item # Item Name Quantity Price
-
RAPID AND MULTITEST IDENTIFICATION SYSTEMS
- -
RM-01 Enterotube multitest system complete with directions ea tube $12.50
RM-02 API microtube strip system complete with directions ea. strip $12.50

NOTE: One tube or one strip is sufficient for demonstrating the respective system per class. Each tube or strip tests one organism. If you wish to demonstrate reactions of more than 1 organism, order as many additional tubes or strips as needed. Additional reagents required are listed below. Quantities shown are sufficient for up to 50 tests.
Item # Item Name Quantity Price
-
For Enterotube System:
- -
RM-03 Kovacs reagent 5.0 ml $4.00
RM-04 Potassium hydroxide 5.0 ml $4.00
RM-05 Alpha naphthol solution 5.0 ml $4.00
RM-08 Oxidase test reagent 5.0 ml $6.00
-
For API System:
- -
RM-06 Sterile mineral oil 10.0 ml $2.00
RM-03 Kovacs reagent 5.0 ml $5.00
RM-07 10% Ferric chloride 5.0 ml $4.00
RM-04 Potassium hydroxide (20%) 5.0 ml $4.00
RM-05 Alpha naphthol solution 5.0 ml $6.00

11. ANTIBIOTIC DISKS FOR SENSITIVITY TESTING


The purpose of this test, in an educational setting, is to demonstrate to the student why this test is necessary and how the test is performed. In order to impart this knowledge, a set of 4-5 different antibiotic disks is sufficient. Students first swab the plates with the test organisms and then place the disks on plates with flame sterilized forceps. You can also use multidisk dispensers but they are not essential for teaching the concept. However, if funds allow it, it is advisable to procure one dispenser so that students can see how this important test is routinely performed in hospitals.

The recommended manual method for antibiotic sensitivity testing is the Kirby-Bauer method. Laboratory handouts describing this procedure including a laboratory exercise are available from this Institute. For ordering information, see below.

While the choice of antibiotic discs can vary, Gram positive organisms are generally tested using the following antibacterial agents:
Item # Item Name Quantity Price
A-01 Clindamycin (2 mcg) ea. $10.50
A-02 Lincomycin (2 mcg) ea. $10.50
A-03 Erythromycin (15 mcg) ea. $10.50
A-04 Penicillin G (10 units) ea. $10.50
A-05 Oxacillin (1 mcg) ea. $10.50
A-06 Methicillin (5 mcg) NO LONGER AVAILABLE ea. -
A-07 Cephalothin (30 mcg) ea. $10.50
A-08 Tetracycline (30 mcg) ea. $10.50
A-09 Gentamicin (10 mcg) ea. $10.50
-
Likewise, Gram negative organisms are generally tested using the following antibacterial agents:
- -
A-10 Ampicillin (10 mcg) ea. $10.50
A-07 Cephalothin (30 mcg) ea. $10.50
A-08 Tetracycline (30 mcg) ea. $10.50
A-09 Gentamicin (10 mcg) ea. $10.50
A-11 Carbenicillin (100 mcg) ea. $10.50
A-12 Kanamycin (30 mcg) ea. $10.50
A-13 Tobramycin (10 mcg) ea. $10.50
A-14 Amikacin (30 mcg) ea. $10.50
A-15 Nitrofurantoin (300 mcg) ea. $10.50
A-16 Trimethoprim (5 mcg) ea. $10.50
A-17 Sulfamethoxazole (.25 mcg) ea. $10.50
A-18 Laboratory handout. A basic guide to the selection and use of antibiotics. Includes a laboratory exercise on sensitivity testing by the Kirby Bauer method One $3.50

12. IMMUNOLOGICAL AND SEROLOGICAL REAGENTS


Major breakthroughs in microbiology and for that matter in biology since 1960 have been in the field of immunology and serology. Concepts and skills, especially methodology, of these important areas are, therefore, essential to providing a complete and up-to-date picture of microbiology and biology. Many of these techniques also form the basis of biotechnology and genetic engineering making their learning even more important.

Unfortunately, reagents used in these areas are produced for use by clinical laboratories and, therefore, are not only expensive, but are often hard to find. An answer to this dilemma is Guaranteed Classroom Quality™ immunological reagents, which are relatively inexpensive. This catalogue lists such reagents and thus opens ways for instructors to teach a variety of antigen-antibody reactions, such as the precipitation reaction, the agglutination reaction, the complement fixation reaction, the neutralization reaction, the hemagglutination reaction, the passive hemagglutination reaction, immuno-double diffusion or the Ouchterlony reaction and immunoelectrophoresis. Simultaneously, an instructor can also show how these tests are used in the design of serological tests for the diagnosis of disease, for testing the purity of proteins and other antigens and in medicolegal areas such as forensics.

In addition to providing serological reagents suitable for teaching, the Institute is also available to assist you in the design and implementation of immunological and serological experiments for your classes. So, feel free to call the Institute with your questions and comments.

DETAILED LABORATORY HANDOUTS Explaining the principles and procedures of immunological and serological reactions are also available from the Institute. For price information, please refer to the end of this section.
Item # Item Name Quantity Price
-
REAGENTS FOR AGGLUTINATION REACTION - with directions
- -
IM-01 Killed, standardized suspension of Salmonella typhosa 5.0 ml $7.50
IM-02 Killed, standardized suspension of Salmonella Montevideo 5.0 ml $7.50
IM-03 Suspension of Salmonella typhosa marked as unknown #1 5.0 ml $7.50
IM-04 Suspension of Salmonella montevideo marked as unknown #2 5.0 ml $7.50
IM-05 Suspension of Salmonella cholerae-suis marked as unknown #3 5.0 ml $7.50
- (Use of these suspensions as unknowns enables students to understand the usefulness of agglutination reactions and also to test their ability to read and differentiate between a positive and a negative agglutination reaction.) - -
IM-06 Anti Salmonella typhosa 5.0 ml $12.50
IM-07 Anti Salmonella Montevideo 5.0 ml $12.50
IM-08 Anti Salmonella cholerae-suis 5.0 ml $12.50
-
REAGENTS FOR PRECIPITATION REACTION ALSO FOR IMMUNODOUBLE DIFFUSION, ELECTROPHORESIS AND IMMUNOELECTROPHORESIS - with directions
- -
IM-09 Human serum 5.0 ml $12.50
IM-10 Goat serum 5.0 ml $8.50
IM-11 Horse serum 5.0 ml $8.50
IM-12 Human gamma globulin 5.0 ml $20.00
IM-13 Human albumin 5.0 ml $10.00
IM-14 Anti human serum 5.0 ml $20.00
IM-15 Anti goat serum 5.0 ml $20.00
IM-16 Anti horse serum 5.0 ml $20.00
- For supplies for Ouchterloney double diffusion, electrophoresis and immuno-electrophoresis,
refer to the Section on Special supplies and reagents for Immunological reactions.
- -
-
REAGENTS FOR NEUTRALIZATION REACTION
- -
-
a. Bacteriophage and antibacteriophage system - with directions
- -
VL-01 Suspension of E. coli T4 bacteriophage 2.0 ml $10.00
IM-11 Anti E. coli T4 bacteriophage 5.0 ml $20.00
LB-33 E. coli strain B one $5.00
- b. Toxin and antitoxin system - with directions
IM-18 Bacterial filtrate containing hemolytic factors 3.0 ml $15.00
IM-19 Antihemolytic factors 3.0 ml $20.00
IM-19A Blood plate for hemolytic reaction one $2.50
-
REAGENTS FOR PASSIVE HEMAGGLUTINATION REACTION - with directions
- -
IM-20 Soluble antigen coupled to red blood cells 5.0 ml $25.00
IM-21 Red blood cells without soluble antigen 5.0 ml $10.00
IM-22 Anti soluble antigen 5.0 ml $20.00
IM-23 Soluble antigen solution 5.0 ml $20.00
-
REAGENTS FOR COMPLEMENT FIXATION REACTION - sufficient for 30 students
- with directions
--
IM-24 Antigen suspension 3.0 ml $10.00
IM-25 Positive control serum for complement fixation 5.0 ml $15.00
IM-26 Negative control serum for complement fixation 5.0 ml $10.00
IM-27 Unknown serum - for complement fixation 5.0 ml $15.00
IM-28 Complement - for complement fixation 5.0 ml $25.00
IM-29 Sheep blood for complement fixation 10.0 ml $20.00
IM-30 Anti-sheep red blood cells for complement fixation 3.0 ml $20.00
IM-31 Veronal Buffer for complement fixation 250 ml $15.00
-
REAGENTS FOR BLOOD TYPING
- -
IM-32 Anti-A serum 5.0 ml $7.50
IM-33 Anti-B serum 5.0 ml $7.50
IM-34 Anti-Rh serum 5.0 ml $20.00
- NOTE: We do not recommend students using their own blood for blood typing. The following
blood cells and unknown blood cell suspensions are, therefore, provided. Quantities are
sufficient for a class of up to 30 students:
--
IM-35 Human red blood cells from type A blood 5.0 ml $15.00
IM-36 Human red blood cells from type B blood 5.0 ml $15.00
IM-37 Human red blood cells from Rh positive blood 5.0 ml $15.00
IM-38 Human red blood cells marked as unknown #1 5.0 ml $15.00
IM-39 Human red blood cells marked as unknown #2 5.0 ml $15.00
IM-40 Human red blood cells marked as unknown #3 5.0 ml $15.00
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REAGENTS FOR SEROLOGICAL TESTS FOR SYPHILIS - with directions
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IM-41 VDRL antigen (0.5 ml +4.5 ml VDRL Buffer) 5.0 ml $20.00
IM-42 RPR antigen 3.0 ml $25.00
IM-43 Positive serum for VDRL or RPR Tests 3.0 ml $20.00
IM-44 Negative serum for VDRL or RPR Tests 3.0 ml $15.00
IM-45 Unknown serum #1 for VDRL or RPR Tests 3.0 ml $20.00
IM-46 Unknown serum #2 for VDRL or RPR Tests 3.0 ml $20.00
IM-47 Card for RPR Test ea. $1.50
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SPECIAL SUPPLIES AND REAGENTS FOR IMMUNOLOGIC REACTIONS
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IM-48 Special agar deep for Outchterloney double diffusion analysis ea. $2.50
IM-49 Special agar deep for electrophoresis or immunoelectrophoresis ea. $2.50
IM-50 Filter Paper or gauze wicks for electrophoresis ea. $2.50
IM-51 Buffer for electrophoresis 8 oz ea. $15.00
IM-52 Washing solution to remove unbound protein for Ouchtorloney and Immunoelectrophoresis ea. $20.00
IM-53 Staining solution for agar gels ea. $20.00
IM-54 De-staining or clearing solution for agar gels ea. $15.00
IM-55 Agar Coated slides for electrophoresis and immunoelectrophoresis Doz. $24.00
IM-56 Blood plate for neutralization reaction for toxin-antitoxin systems ea. $2.50
IM-57 Precipitin tubes (6 X 50 mm) ea. $0.75
IM-58 Capillary tubes for precipitation reactions ea. $0.75
IM-59 LABORATORY HANDOUTS on principles and procedures of immunological and serological reactions ea. $5.00
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SUPPLIES FOR ENVIRONMENTAL,INDUSRIAL, SANITARY, FOOD AND DAIRY TESTING
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SUPPLIES FOR WATER TESTING:
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- Conventional water testing method: --
PM-28 28 Lactose broth single strength ea. $1.25
PM 28 Lactose broth double strength ea. $1.50
PM 24 EMB (Eosin Methylene Blue) agar plate ea. $0.85
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Membrane Filter Method:
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WT-1 Syringe 50 ml Luerlock Plastic disposable ea.
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WT-2 Membrane filter holder 47 mm autoclavable ea. $35.00
WT-3 Membrane filters 0.45 microns, 47 mm nonsterile autoclavable pack of 100 ea. $65.00
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WT-4 Petri plate 50 mm with 47 mm absorbent pad ea. $2.00
WT-5 Endos broth 5.0 ml ea. $1.00
WT-6 Total count broth 5.0 ml ea. $1.00
WT-7 Coliform count broth 2.0 ml ea. $1.50
WT-8 Media for fungi and moulds 2.0 ml ea. $1.50
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SUPPLIES FOR INDUSTRIAL MICRO BIOLOGY (FOOD, PHARMACEUTICALS AND COSMETICS) :
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Sterility Testing (for Pharmaceuticals and Cosmetics) :
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ST-1 Thioglycolate broth 15 ml. (20 x 150) mm tubes ea. $1.00
ST-2 Thioglycolate broth 40 ml. (20 x 150) mm tubes ea. $1.50
ST-3 Thioglycolate broth 100 ml. (20 x 150) mm tubes ea. $2.50
LB-79 Staphylococcus epidermidis ea. $5.00
LB-80 Staphylococcus aureus ea. $5.00
LF-101 Candida albicans ea. $7.50
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Viable Count (for Food, Pharmaceuticals and Cosmetics) :
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VC-1 Saline diluent blanks sterile ea. $0.75
VC-2 Total count media, 100ml bottle ea. $10.00
VC-3 Total count media, 250ml bottle ea. $15.00
MM-23 Petri plates sterile disposable 15 x100 mm box of 500 ea. $80.00
MM-47 Petri plates sterile disposable 15 x100 mm sleeve of 20 ea. $5.00
MM-50 Pipettes sterile disposable 1 ml ea. $0.35
MM-51 Pipettes sterile disposable 10 ml ea. $0.60
- SUPPLIES FOR DAIRY PRODUCTS: --
- Milk testing: --
DT-1 Methylene blue thiocyanate solution 50 ml ea. $10.00
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SUPPLIES FOR COSMETICS:
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CC-1 Modified Letheen broth 50 ml ea. $1.50
CC-2 Modified Letheen Agar 50 ml ea. $1.50

Our History

The Center for Integrative Learning has been teaching students of all ages and creeds the fundamentals of Microbiology and lab sciences for over five decades.
The center is located at 729 S Western, has over 14,000 square feet of lab space for teaching, and is considered a one-of-a-kind facility that exceeds education standards worldwide.